Design and Synthesis of a Quenched Fluorogenic Peptide Substrate for Human Cytomegalovirus Proteinase-Reference-Cited by-同舟云学术

Design and Synthesis of a Quenched Fluorogenic Peptide Substrate for Human Cytomegalovirus Proteinase

Published:1995-08 Issue:4 Volume:6 Page:255-261
ISSN:2040-2066
Container-title:Antiviral Chemistry and Chemotherapy
language:en
Short-container-title:Antivir Chem Chemother

Author:

Handa B.K.¹,Keech E.¹,Conway E.A.¹,Broadhurst A.²,Ritchie A.²

Affiliation:

1. Department of Physical Methods, Roche Products Limited, PO Box 8, Welwyn Garden City, Hertfordshire AL7 3AY, UK

2. Department of Biology, Roche Products Limited, PO Box 8, Welwyn Garden City, Hertfordshire AL7 3AY, UK

Abstract

A fluorogenic peptide substrate for HCMV proteinase was synthesized by solid-phase peptide synthesis. The amino acid sequence of this substrate is derived from the maturation cleavage site (M site) of the natural substrate, the assembly protein precursor. The minimum sequence for efficient cleavage requires at least seven residues (P4-P3′). A systematic modification of the peptide substrate was carried out to identify positions suitable for the introduction of the fluorescent donor and the quencher acceptor groups.

Publisher

SAGE Publications

Link

http://journals.sagepub.com/doi/pdf/10.1177/095632029500600408

Reference17 articles.

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