In-vitro anti Myxovirus Activity and Mechanism of Antiinfluenzavirus Activity of Polyoxometalates PM-504 and PM-523

Author:

Shigeta S.1,Mori S.1,Watanabe J.1,Yamase T.2,Schinazi R. F.3

Affiliation:

1. Department of Microbiology, Fukushima Medical College, Fukushima 960-12, Japan

2. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama 227, Japan

3. Veterans Affairs Medical Centre and Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA

Abstract

Sixty polyoxometalates were examined for anti-influenza A virus (FluV-A) activity in vitro. Two of the most potent and least cytotoxic compounds, PM-504 K9H5(Ge2Ti6W18O77)16H2O] and PM-523 (iPrNH3)6H [PTi2W10O38(O2)9H2O2] were selected for further studies. Examination of the antiviral effects of PM-504 and PM-523 against other human ortho- and paramyxoviruses revealed that both compounds had broad spectrum antimyxovirus activities. From a time of addition study and FACS analysis for influenza A virus infected cells, the compounds were found not to inhibit binding of virus to MDCK cells. However, these compounds inhibited haemolysis of chicken erythrocytes by virus and also inhibited fluorescence dequenching of octadecylrhodamine B-labelled virus after binding to cells. This indicates that these polyoxometalates inhibited fusion of the virus envelope to the cellular membrane.

Publisher

SAGE Publications

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