The Use of Antisera to the Human Herpesvirus 6 Major Capsid Protein to Determine the Effect of Antiviral Inhibitors on Virus Gene Expression-Reference-Cited by-同舟云学术

The Use of Antisera to the Human Herpesvirus 6 Major Capsid Protein to Determine the Effect of Antiviral Inhibitors on Virus Gene Expression

Published:1990-10 Issue:5 Volume:1 Page:313-318
ISSN:2040-2066
Container-title:Antiviral Chemistry and Chemotherapy
language:en
Short-container-title:Antivir Chem Chemother

Author:

Liu M.-Y.¹,Arrand J. R.¹,Littler E.¹

Affiliation:

1. Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Wilmslow Road, Manchester M20 9BX, UK

Abstract

We have recently described the production of antisera which react with the human herpesvirus 6 (HHV-6) major capsid protein (MCP). We describe here the use of these specific antisera to characterize the kinetics of expression of the HHV-6 MCP and demonstrate that it is a late virus protein, expressed after DNA replication. We have used the expression of this protein as a measure of the efficacy of the antiviral nucleoside analogues 9-(2-hydroxyethoxymethyl)guanine (ACV) and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) upon HHV-6 replication and have determined 50% inhibitory concentrations to be 150 μm and 2 μm, respectively.

Publisher

SAGE Publications

Link

http://journals.sagepub.com/doi/pdf/10.1177/095632029000100506

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