Antiherpesvirus Activity of the Combination of 5-iodo-2′-deoxycytidine with methotrexate: An in vitro and in vivo Study-Reference-Cited by-同舟云学术

Antiherpesvirus Activity of the Combination of 5-iodo-2′-deoxycytidine with methotrexate: An in vitro and in vivo Study

Published:1994-10 Issue:5 Volume:5 Page:283-289
ISSN:2040-2066
Container-title:Antiviral Chemistry and Chemotherapy
language:en
Short-container-title:Antivir Chem Chemother

Author:

Cremonesi C.¹,Scarpini C.¹,Bianchi R.²,Radaelli A.¹³,Gimelli M.¹,De Giuli Morghen C.¹

Affiliation:

1. Department of Pharmacology and CNR Center for Cytopharmacology, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy

2. CNR Center for the Study of the Relations between Structure and Chemical Reactivity, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy

3. Institute of Pharmacological Sciences, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy

Abstract

We evaluated the in vitro and in vivo antiviral activity of the deoxyribonucleoside analogue 5-iodo-2′-deoxycytidine (IDC) combined with the dihydrofolate reductase inhibitor methotrexate (MTX) on herpes simplex virus types 1 and 2 (HSV-1, HSV-2). The IDC-MTX combination synergistically inhibited HSV-1 and HSV-2 replication in vitro at concentrations that did not reduce cellular viability and was very effective in reducing the severity of cutaneous lesions in the experimental guinea pig model in vivo. The antiviral activity of the IDC-MTX combination in guinea pigs was also compared with that of acyclovir and was demonstrated to be higher.

Publisher

SAGE Publications

Link

http://journals.sagepub.com/doi/pdf/10.1177/095632029400500501

Reference26 articles.

1. Methylation of HSV-1 DNA as a mechanism of viral inhibition: Studies of an analogue of methyldeoxycytidine: Trifluoromethyldeoxycytidine (F3mdCyd)

2. Deoxyribonucleotide metabolism in herpes simplex virus infected HeLa cells

3. Roles of thymidylate synthetase activity in herpes simplex virus-infected HeLa cells

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Three-dimensional analysis of combination effect of ellagitannins and acyclovir on herpes simplex virus types 1 and 2;Antiviral Research;2011-02