Anionic Compounds as Inhibitors of African Swine Fever Virus Replication in Vero Cells

Abstract

The anionic compounds, aurintricarboxylic acid, Evans blue, glycyrrhizic acid, taurolithocholic acid, and fuchsin acid, have been tested for their capacity to inhibit the replication of African swine fever virus (ASFV) in Vero cells. Aurintricarboxylic acid suppressed ASFV cytopathic effects at 5μg ml−1 and emerged as the most efficacious inhibitor with a selectivity index (S.I.) of 400, followed by Evans blue with 40, Fuchsin acid with 20, taurolithocholic acid with 10, and glycyrrhizic acid with 5. All the compounds inhibited the virus adsorption or penetration to the cells in some degree, but inhibition was also found when they were added after virus adsorption, indicating that other mechanism (or mechanisms) are involved in viral inhibition.