Assay for Ubiquitin Ligase Activity: High-Throughput Screen for Inhibitors of HDM2-Reference-Cited by-同舟云学术

Assay for Ubiquitin Ligase Activity: High-Throughput Screen for Inhibitors of HDM2

Published:2004-12 Issue:8 Volume:9 Page:695-703
ISSN:1087-0571
Container-title:Journal of Biomolecular Screening
language:en
Short-container-title:J Biomol Screen

Author:

Davydov I. V.¹,Woods D.²,Safiran Y. J.,Oberoi P.¹,Fearnhead H. O.³,Fang S.⁴,Jensen J. P.,Weissman A. M.³,Kenten J. H.¹,Vousden K. H.⁵

Affiliation:

1. Meso-Scale Discovery, Meso-Scale Diagnostics, LLC, Gaithersburg, MD.

2. Regulation of Cell Growth Laboratory and Laboratory of Protein Dynamics and Signaling, NCI Center for Cancer Research, Frederick; Department of Antibody Development, Human Genome Science, Gaithersburg, MD.

3. Regulation of Cell Growth Laboratory and Laboratory of Protein Dynamics and Signaling, NCI Center for Cancer Research, Frederick, MD.

4. Regulation of Cell Growth Laboratory and Laboratory of Protein Dynamics and Signaling, NCI Center for Cancer Research, Frederick; Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD.

5. Regulation of Cell Growth Laboratory and Laboratory of Protein Dynamics and Signaling, NCI Center for Cancer Research, Frederick, MD; Beatson Institute for Cancer Research, Gascube Estate, Switchback Road, Glasgow, G61 1BD, UK

Abstract

An assay for the autoubiquitination activity of the E3 ligaseHDM2 (Mdm2) was developed and adapted to a high-throughput format to identify inhibitors of this activity. The assay can also be used tomeasure the activity of other E3s andmay be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.

Publisher

Elsevier BV

Link

http://journals.sagepub.com/doi/pdf/10.1177/1087057104267956

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