SpeedScreen: The “Missing Link” between Genomics and Lead Discovery-Reference-Cited by-同舟云学术

SpeedScreen: The “Missing Link” between Genomics and Lead Discovery

Published:2004-09 Issue:6 Volume:9 Page:498-505
ISSN:1087-0571
Container-title:Journal of Biomolecular Screening
language:en
Short-container-title:J Biomol Screen

Author:

Zehender Hartmut¹,Le Goff Francois²,Lehmann Natalie³,Filipuzzi Ireos⁴,Mayr Lorenz M.⁵

Affiliation:

1. Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.208

2. Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland

3. Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.209

4. Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.114

5. Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland; WSJ-350.102

Abstract

SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.

Publisher

Elsevier BV

Link

http://journals.sagepub.com/doi/pdf/10.1177/1087057104267605

Reference5 articles.

1. The Sequence of the Human Genome

2. SpeedScreen: label-free liquid chromatography–mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands

3. Instrumentation, applications, and energy deposition in quadrupole ion-trap tandem mass spectrometry

4. Staurosporine-induced conformational changes of cAMP-dependent protein kinase catalytic subunit explain inhibitory potential

5. THE UBIQUITIN SYSTEM

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