A High-Throughput Soft Agar Assay for Identification of Anticancer Compound-Reference-Cited by-同舟云学术

A High-Throughput Soft Agar Assay for Identification of Anticancer Compound

Published:2007-10 Issue:7 Volume:12 Page:938-945
ISSN:1087-0571
Container-title:Journal of Biomolecular Screening
language:en
Short-container-title:J Biomol Screen

Author:

Anderson Steven N.¹,Towne Danli L.¹,Burns David J.¹,Warrior Usha²

Affiliation:

1. Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL

2. Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL,

Abstract

A 384-well soft agar assay was developed to identify potential novel anticancer compounds. Normally used to detect cell transformation, the assay is used here to quantitate cell proliferation in a 3-dimensional (3-D) anchorage-independent format. HCC827 cells, which are highly sensitive to epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors, were used to develop the method and a set of 9600 compounds used to validate the assay. Results were compared to a monolayer assay using the same compound set. The assay provides a robust method to discover compounds that could be missed using traditional monolayer formats. ( Journal of Biomolecular Screening 2007:938-945)

Publisher

Elsevier BV

Link

http://journals.sagepub.com/doi/pdf/10.1177/1087057107306130

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