Interstitial expression of angiotensin II and AT1 receptor are increased in patients with progressive glomerulopathies

Abstract

In animal models, interstitial angiotensin II (ang II) and AT1 receptor (AT1R) are key mediators of renal inflammation and fibrosis in progressive chronic nephropathies. We hypothesized that these molecules were overexpressed in patients with progressive glomerulopathies. In this observational retrospective study, we described the expression of ang II and AT1R by immunohistochemistry in kidney biopsies of 7 patients with minimal change disease (MCD) and in 25 patients with progressive glomerulopathies (PGPs). Proteinuria, serum albumin, and serum creatinine were not statistically different between MCD and PGP patients. Total expression of ang II and AT1R was not statistically different between MCD (108.7±11.5 and 73.2±13.6 cells/mm2, respectively) and PGN patients (100.7±9.0 and 157.7±13.8 cells/mm2, respectively; p>0.05). Yet, interstitial expression of ang II and AT1R (91.6±16.0 and 45.6±5.4 cells/mm2, respectively) was higher in patients with PGN than in those with MCD (22.0±4.1 and 17.9±2.9 cells/mm2, respectively, p<0.05), as was the proportion of interstitial fibrosis (11.0±0.7% versus 6.1±1.2%, p<005). In patients with MCD, ang II and AT1R expressions predominate in the tubular compartment (52% and 36% of the positive cells, respectively). In those with PGP, the interstitial expression of ang II and AT1R predominates (58% and 45%, respectively). In conclusion, interstitial expression of ang II and AT1R is increased in patients with progressive glomerulopathies. The relationship of these results and interstitial fibrosis and disease progression in humans warrants further investigations.