Anti-inflammatory Activity of Melampyrum barbatum and Isolation of Iridoid and Flavonoid Compounds-Reference-Cited by-同舟云学术

Anti-inflammatory Activity of Melampyrum barbatum and Isolation of Iridoid and Flavonoid Compounds

Published:2018-03 Issue:3 Volume:13 Page:1934578X1801300
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Háznagy-Radnai Erzsébet¹,Fási Laura¹,Wéber Edit²,Pinke Gyula³,Király Gergely⁴,Sztojkov-Ivanov Anita⁵,Gáspár Róbert⁵,Hohmann Judit¹⁶

Affiliation:

1. Department of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary

2. Institute of Pharmaceutical Analysis, University of Szeged, Eötvös u. 6, 6720, Szeged, Hungary

3. Department of Botany, Faculty of Agricultural and Food Sciences, Széchenyi István University, Vár 2, H-9200 Mosonmagyaróvár, Hungary

4. Department of Sylviculture, Institute of Sylviculture and Forest Protection, University of Sopron, Bajcsy-Zsilinszky Endre u. 4, H-9400, Sopron, Hungary

5. Department of Pharmacodynamics and Biopharmacy, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary

6. Interdisciplinary Centre of Natural Products, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary

Abstract

Melampyrum barbatum Waldst. & Kit. ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1801300301

Reference13 articles.

1. The Herbal DrugMelampyrum pratenseL. (Koch): Isolation and Identification of Its Bioactive Compounds Targeting Mediators of Inflammation

2. Iridoid glucosides from Melampyrum

3. Pyrrolizidine alkaloids fromMelampyrum pratense

4. Identification of Iridoids, Flavonoids and Triterpenes from the Methanolic Extract of Melampyrum bihariense A. Kern. and the Antioxidant Activity of the Extract