Antimicrobial Activities of a New Alkaloid from Marine-Derived Fungus, Penicillium sp. OM01

Abstract

Objectives This study focused on the isolation, structural elucidation, and antimicrobial activities of compounds from the culture broth of the strain Penicillium sp. OM01 (isolated from the marine mollusk sample Spondylus squamosus (Schreibers, 1793)). Methods Various chromatographic methods were used to isolate compounds from the ethyl acetate and MeOH extracts of the strain Penicillium sp. OM01. Their structures were elucidated using HR-ESI-MS and NMR data. The compounds were evaluated for antimicrobial effects using the dilution turbidimetric broth method. Results One new alkaloid, penicritin (1), together with seven known compounds, including phenol A acid (2), topsentisteron D3 (3), ergostanol (4), (22 E,24 R)-24-methylcholesta-5,22-diene-3 β, 7 β-diol (5), 5 α,6 α-epoxy-(22 E,24 R)-ergosta-8,22-diene-3 β,7 α-diol (6), staphylopeptide A (7), and 4-hydroxybenzoic acid (8) were isolated from the culture broth of the marine-derived fungus Penicillium sp. OM01. Compounds 2-4 and 6 exhibited antibacterial activity against Gram-(+) bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, and yeast Candida albicans with MIC values ranging from 32 to 256 µg/mL. Conclusions This is the first report of the strain Penicillium sp. OM01 from the marine mollusk sample S. squamosus (Schreibers, 1793). One new and seven known compounds were isolated from the strain Penicillium sp. OM01 (isolated from the marine mollusk sample S. squamosus (Schreibers, 1793). Compound 4 is a potential antibacterial agent.