Optimization of Microwave-Assisted Extraction of Phlorotannin From Sargassum swartzii (Turn.) C. Ag. With Ethanol/Water

Author:

Toan Tran Quoc12,Phong Tran Duy12,Tien Dam Duc234,Linh Nguyen Manh23ORCID,Mai Anh Nguyen Thi3,Hong Minh Pham Thi12,Duy Le Xuan12,Nghi Do Huu2,Pham Thi Hai Ha56,Nhut Pham Tri56,Tung Ho Sy7,Tung Nguyen Quang7

Affiliation:

1. Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

3. Hai Phong Institute of Oceanic Resources, Vietnam Academy of Science and Technology, Hai Phong, Vietnam

4. Hai Phong University of Medicine and Pharmacy, Hai Phong, Vietnam

5. NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

6. Center of Excellence for Biochemistry and Natural Products, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

7. Hanoi University of Industry, Hanoi, Vietnam

Abstract

Sargassum is a genus of brown macroalgae in the class Phaeophyta, distributed widely in all oceans, including those of Vietnam. Species of this genus have been proven to possess diverse biological activities, such as antioxidant, anti-fungal, and anti-inflammatory, along with many benefits and applications for human health, including anti-diabetic, obesity, and thrombosis. These benefits arise from a diverse chemical composition, with compounds such as fucoidan, mannitol, and especially phlorotannin—a group of phenolic derivatives found predominantly in brown algae. In this study, we evaluated and optimized the factors that affected the extraction process of phlorotannins from Sargassum swartzii (Turn.) C. Ag., a common species of brown macroalgae in Vietnam. The process utilized ethanol and water as the solvent system, and the extraction process was assisted with the use of microwaves. To carry out optimization studies, Response Surface Methodology (RSM) was adopted according to a Central Composite Desisgn (CCD), taking four processing factors into consideration, ethanol concentration (%, v/v), extraction time (minutes), solvent/material ratio (v/w), and microwave output power (W) as independent variables. Phlorotannin concentration (mgPhE/g) and extract mass (mg) were regarded as optimization outcomes. Experimental conditions that produced the highest phlorotannin yield from 10 g of S. swartzii are as follows: Extraction time of 65 minutes, ethanol concentration of 52%, microwave output power of 613 W, and solvent/material ratio of 33/1 (v/w). These conditions corresponded to a phlorotannin concentration of 5.59 ± 0.11 mg PhE/g, and a total extract content of 27.88 ± 0.13 mg/g.

Funder

vietnam academy of science and technology

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3