Isolation a Homogalacturonan from the Outer Seed Coat of Shaddock (Citrus grandis Osbeck)-Reference-Cited by-同舟云学术

Isolation a Homogalacturonan from the Outer Seed Coat of Shaddock (Citrus grandis Osbeck)

Published:2018-06 Issue:6 Volume:13 Page:1934578X1801300
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Wang Heng Long¹²,Tu Chin Wei¹,Wu Wei Zhi¹,Lin Chen Yi¹,Chen Su Yu¹,Yang Wen Jen¹²,Chang Jer Chia³,Lu Mei Kuang⁴,Liao Wei Tung⁵

Affiliation:

1. Department of Life Sciences, National University of Kaohsiung, No. 700 Kaohsiung University Rd. Nan-Tzu District 811, Kaohsiung, Taiwan

2. Biotechnology Research Center, National University of Kaohsiung, No. 700 Kaohsiung University Rd. Nan-Tzu District 811, Kaohsiung, Taiwan

3. Department of Horticulture, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan

4. National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan 155-1 Li-Nung St., Sec. 2, Beitou 112, Taipei, Taiwan

5. Department of Chemical and Materials Engineering, Southern Taiwan University of Science and Technology, No. 1, Nan-Tai Street, Yungkang District 710, Tainan, Taiwan

Abstract

An acidic mucilage was isolated from the outer-layer seed coat of shaddock (Citrus grandis Osbeck) by water extraction at room temperature, and purified by DE-52 anion-exchange chromatography using 0.26 – 0.37 M NaCl. This purified mucilage was almost entirely composed of galacturonic acid residues. Glycosyl-linkage analysis showed that the backbone was 1→4 linked. Fourier Transform Infrared Spectroscopic analysis and determination of the degree of methylation further revealed that the mucilage was a low-degree (11.94%) esterified homogalacturonan. Size exclusion chromatography showed that the major molecular weight distribution of 610.9 kDa.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1801300625

Reference24 articles.

1. Cytology of mucilage production in the seed coat of Candle canola (Brassica campestris)

2. Polysaccharide hydrolases are released with mucilages after water hydration of flax seeds

3. The structure, function, and biosynthesis of plant cell wall pectic polysaccharides

4. Novel Rhamnogalacturonan I and Arabinoxylan Polysaccharides of Flax Seed Mucilage

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