A Rapid Study of Botanical Drug–Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin–Functionalized Magnetic Nanoparticles-Reference-Cited by-同舟云学术

A Rapid Study of Botanical Drug–Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin–Functionalized Magnetic Nanoparticles

Published:2015-12 Issue:12 Volume:10 Page:1934578X1501001
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Qing Lin-Sen¹,Xue Ying¹,Ding Li-Sheng¹,Liu Yi-Ming²,Liang Jian¹,Liao Xun¹

Affiliation:

1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China, 610041

2. Jackson State University, Mississippi, USA, 39217

Abstract

A great many active constituents of botanical drugs bind to human serum albumin (HSA) reversibly with a dynamic balance between the free- and bound-forms in blood. The curative or side effect of a drug depends on its free-form level, which is always influenced by other drugs, combined dosed or multi-constituents of botanical drugs. This paper presented a rapid and convenient methodology to investigate the drug-drug interactions with HSA. The interaction of two steroidal saponins, dioscin and pseudo-protodioscin, from a botanical drug was studied for their equilibrium time and equilibrium amount by re-ligand fishing using HSA functionalized magnetic nanoparticles. A clear competitive situation was obtained by this method. The equilibrium was reached soon about 15 s at a ratio of 0.44: 1. Furthermore, the interaction of pseudo-protodioscin to total steroidal saponins from DAXXK was also studied. The operation procedures of this method were faster and more convenient compared with other methods reported.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1501001237

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