Biotransformation to Produce the Anticancer Compound Colchicoside Using Cell Suspension Cultures of Astragalus vesicarius Plant Species

Abstract

This paper discusses the biotechnological process affected by means of plant suspension cultures, for production of colchicoside, the 3- O-glucosyl derivative of 3- O-demethylcolchicine. Colchicoside can be considered as an antitumoural prodrug which is activated after oral administration and may have more beneficial effects and a better toxicity profile (because of a slow-release effect) than colchicine. We have developed a green and efficient biotechnological method using colchicine, as a precursor, derived from its natural source G. superba seeds. Plant suspension cultures of Astragalus vesicarius were used to design a practical biotechnological platform to replace a methyl group at C-3 regiospecifically by a glycosyl moiety in colchicine. Using different concentrations of a colchicine-rich extract, the maximum enzymatic potential of Astragalus vesicarius suspension cells was achieved. According to quantitative HPLC-UV analysis, levels of 9.35 μmol/g DW colchicoside were achieved. This is the first report of region-specific glycosylation at C-3 of the aromatic ring A of the colchicine using plant suspension cultures.