Chemical Constituents From Endiandra kingiana (Lauraceae) as Potential Inhibitors for Dengue Type 2 NS2B/NS3 Serine Protease and its Molecular Docking

Abstract

A phytochemical study on the bark of Endiandra kingiana Gamble (Lauraceae) led to the isolation of a new benzofuranone, 4-hydroxy-6-(9,13,17-trimethyldodeca-8,12,16-trienyl)-2(3 H)-benzofuranone (1), together with 6 known compounds. Their structures were established on the basis of detailed spectroscopic analysis, including one- and two-dimensional nuclear magnetic resonance (NMR) and electrospray Ionisation mass spectrometry techniques. Compounds 1-3 showed moderate inhibition against dengue virus type 2 NS2B/NS3 protease with percentage inhibitions of 61.23 ± 6.96, 69.92 ± 3.34, and 62.02 ± 6.19, respectively. Molecular docking was performed to predict the binding mode of all protease inhibitor models and the results revealed that most of the essential amino acid residues such as Asp129, Ser135, Tyr161, Asn152, and His51 significantly interact with the ligands.