Natural Indoles From the Bacterium Pseudovibrio denitrificans P81 Isolated From a Marine Sponge, Aaptos Species

Author:

Fang Shu-Yen12,Chen Sheng-Yuan3,Chen You-Ying45,Kuo Tsu-Jen467,Wen Zhi-Hong48,Chen Yu-Hsin5,Hwang Tsong-Long291011,Sung Ping-Jyun45121314ORCID

Affiliation:

1. Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan

2. Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, Taiwan

3. Department of Internal Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan

4. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan

5. National Museum of Marine Biology and Aquarium, Pingtung, Taiwan

6. School of Dentistry, Chung Shan Medical University, Taichung, Taiwan

7. Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan

8. Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan

9. Research Center for Chinese Herbal Medicine, Graduate Institute of Healthy Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan

10. Department of Anaesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan

11. Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan

12. Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan

13. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan

14. Ph.D. Program in Pharmaceutical Biotechnology, Fu Jen Catholic University, New Taipei, Taiwan

Abstract

A new natural indole, vibrindole B (1), together with known analogs, vibrindole A (2), trisindoline (3), norharmane (4), and 3-(hydroxyacetyl)indole (5), produced by the bacterium Pseudovibrio denitrificans P81, were isolated from a sponge, Aaptos species. The structures of indoles 1 to 5 were established by spectroscopic methods. The proposed biosynthetic pathway of 1 to 5 is also discussed, starting from tryptophan. Moreover, indoles 1 to 3 were found to exhibit cytotoxicity toward T24 tumor cells with IC50 values of 1.71 ± 0.11, 4.53 ± 0.14, and 2.26 ± 0.26 µM, respectively.

Funder

Zuoying Branch of Kaohsiung Armed Forces General Hospital

Ministry of Science and Technology, Taiwan

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

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