Affiliation:
1. Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu, China
2. Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu, China
3. Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs; Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, China
Abstract
Nowadays, plant allelopathy, as a new type of biological algal and/or bacterial inhibition technology, has attracted extensive attention. Eight substances were isolated and identified from Jussiaea stipulacea Ohwi, and five concentration gradients, as well as a control (0, 1.25, 5, 10, 20, and 50 mg/L) were set, with three parallels in each group, and then sampled and detected at 24, 48, 72, and 96 h. When the concentration was 50 mg/L, the inhibition rate of Anabaena was as high as 74.8%, 69.2%, and 70.7% for ursolic acid, kaempferol, and luteolin, respectively. Streptococcus iniae and Aeromonas hydrophila were cultured to a logarithmic phase, and their final concentrations reached 1000, 500, 250, 125, 62.50, 31.25, 15.63, and 7.81 μg/mL. Luteolin and gallic acid showed an inhibitory effect on S iniae and A hydrophila at 1000 μg/mL. We found that allelochemicals also had a certain bacteriostatic effect, among which luteolin has great development potential.
Subject
Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine