Exploring the Insect Control and Plant Growth Promotion Potentials of Endophytes Isolated From Calotropis procera Present in Jeddah KSA

Abstract

Pseudomonas bacteria are entomopathogenic that can naturally infect and kill insects upon ingestion. The insecticidal and plant growth-promoting roles of the bacteria were assessed by applying Pseudomonas IUK001 to insects and plants. The culture extract (CE) of IUK001 at the concentration of 1 mL/cm3 of an artificial diet was used as a treatment for Galleria mellonella larvae. The CE was heat stable at 70°C for 30 minutes and proteinase-K stable, showing 100% and 90% insecticidal activity against G. mellonella larvae. Gas chromatography–mass spectrometry (GC–MS) analysis revealed the insecticidal compounds, for example, trans-cinnamic acid, ornithine, and cyclo (l-Pro-l-Val) in the CE of IUK001. Antibacterial activity was assessed through minimal inhibitory concentration and half-maximal inhibitory concentration values, which ranged from 28 ± 0.8 and 18.9 ± 0.7 to 8 ± 1.8 and 4.6 ± 0.6, respectively. Moreover, plants were inoculated with IUK001, which significantly enhanced ( P < 0.05) the total plant lengths (root + shoot), dry and fresh biomasses and chlorophyll content, and also induced lateral roots. Plant growth hormones, auxins: indole-3-butyric acid and indole-3- propionic acid, were then analyzed through GC–MS in the CE using their respective isotopic internal standards. The findings of the study suggest that IUK001 is a significantly valuable candidate to be exploited as a biocontrol agent as well as a plant growth promoter.