Flower Infusions FromCornus masandCornus kousaInhibit Aldose Reductase Enzyme, Without Any Effects on Lipotoxicity

Author:

Forman Vladimír1ORCID,Šušaníková Ivana1,Kukurová Ľubica1,Švajdlenka Emil23,Nagy Milan1,Mučaji Pavel1

Affiliation:

1. Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Slovakia

2. Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Slovakia

3. Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic

Abstract

Aldose reductase inhibitors are considered to be potential therapeutic agents for chronic diabetic complications. Diabetes mellitus can be accompanied by elevated blood levels of free fatty acids, which can cause lipotoxicity. Herbal extracts and their constituents are promising agents which have the potential for alleviating these complications. Our study was focused on the influence on these effects by flower infusions from Cornus mas L. and Cornus kousa F.Buerger ex Hance. Initially, phenolic compounds were quantified in the dried flowers. Next, we studied the ability of flower infusions from both plants to inhibit aldose reductase in vitro, the protective role in the cell model of lipotoxicity, and the cytotoxic action on fibroblast cell line NIH-3T3 by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Both species are rich in phenolics; C. kousa flowers contain slightly higher amounts of phenolic acids (20.8%) and flavonoids (56.1%) than C. mas (20.2%) and (47.4%), respectively. Both extracts showed effective inhibition, expressed as half-maximal inhibitory concentration (IC50) (the concentration of inhibitor required to exhibit 50% inhibition of the enzyme reaction), of aldose reductase in non-toxic low concentrations (IC50= 3.06 μg/mL for C. mas and IC50= 2.49 μg/mL for C. kousa, respectively). In contrast, these concentrations of both extracts had almost no effects in the lipotoxicity cell model. To our knowledge, this study is the first report on C. mas and C. kousa flowers’ aldose reductase inhibitory activity and influence upon lipotoxicity.

Funder

Agentúra Ministerstva školstva, vedy, výskumu a športu SR

Univerzita Komenského v Bratislave, Farmaceutická fakulta

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

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