Environmental Factors do not Affect the Phenolic Profile of Hypericum perforatum Growing Wild in Bosnia and Herzegovina

Author:

Zeljković Sanja Ćavar12,Karalija Erna3,Parić Adisa3,Muratović Edina3,Tarkowski Petr12

Affiliation:

1. Centre of Region Haná for Biotechnogical and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, Slechtitelu 27, 78371 Olomouc, Czech Republic

2. Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Šlechtitelů 29, 78371 Olomouc, Czech Republic

3. University of Sarajevo, Faculty of Science, Department of Biology, Laboratory for research and protection of endemic resources, Zmaja od Bosne 33–35, 71000 Sarajevo, Bosnia and Herzegovina

Abstract

Using appropriate isolation and fractionation procedure, and UHPLC-MS/MS as powerful analytical tool, comprehensive phenolic acid profile of leaves and flowers of Hypericum perforatum L., growing wild in Bosnia and Herzegovina, has been analyzed for the first time. The phenolic acids were isolated from plant tissue, liberated from esters and glycosidic bonds, and then analyzed as free acids. Results show that free forms of caffeic and chlorogenic acid were the most abundant. Generally, the content of acids is higher in flowers than in leaves, but environmental factors do not significantly influence chemical composition of this medicinal plant.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

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