Quantitative and Qualitative Analysis of Eruca sativa and Brassica juncea Seeds by UPLC-DAD and UPLC-ESI-QTOF-Reference-Cited by-同舟云学术

Quantitative and Qualitative Analysis of Eruca sativa and Brassica juncea Seeds by UPLC-DAD and UPLC-ESI-QTOF

Published:2017-09 Issue:9 Volume:12 Page:1934578X1701200
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Sharma Arti¹,Sharma Ritika¹²,Arora Rohit³,Arora Saroj³,Singh Bikram¹²,Sharma Upendra¹²

Affiliation:

1. Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061, Himachal Pradesh, India

2. Academy of Scientific and Innovative Research, CSIR-IHBT, Palampur, Himachal Pradesh 176061, India

3. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India

Abstract

Eruca sativa and Brassica juncea belong to the Brassicaceae family and have been used traditionally for the treatment of cancer and various cardiovascular ailments. A rapid and simple ultra performance liquid chromatography (UPLC) method has been developed for the simultaneous quantification of erucin, allyl isothiocyanate and benzyl isothiocyanate in E. sativa and B. juncea oil. Eruca sativa oil contains erucin (28.93%), allyl isothiocyanate (0.06%) and benzyl isothiocyanate (0.70%). Identification of other secondary metabolites in E. sativa and B. juncea oil and aqueous methanolic extracts were also carried out using ultra performance liquid chromatography-electro spray ionization-quadrupole time of flight (UPLC-ESI-QTOF). The UPLC-ESI-QTOF based study leads to the identification of ten compounds in B. juncea oil and three compounds in E. sativa oil. The UPLC-ESI-QTOF analysis of aqueous methanolic extracts of E. sativa and B. juncea leads to the identification of eight and nine compounds, respectively.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1701200927

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