The Effects of Resveratrol Targeting MicroRNA-4325P/PDGF-B to Regulate Tumor Angiogenesis in Osteosarcoma Microenvironment

Abstract

Objectives Here we sought to determine the specific mechanism by which resveratrol targets microRNA-4325p and PDGFB in an osteosarcoma (OS) cell line and investigate its effect on the OS cell line. Methods As a result of the study related to the effect of resveratrol on the conditioned media of microRNA-4325p mimic transfected MG-63 and HOS (a fibroblast and epithelial-like cell line) cells, we determined that resveratrol decreased survival, and growth, migration, and aortic ring formation of endothelial cells. A combination of miRNA mimics and HOS and MG-63 cell lines were transfected with inhibitors to explore the mechanisms behind angiogenesis formation induced by resveratrol. Results We found that resveratrol improved the activity of microRNA-4325p mimic transfection in OS, thus promoting angiogenesis, as demonstrated by angiogenic parameters and gene expression in OS. Following these results, it is evident that resveratrol can modulate angiogenesis by regulating microRNA-4325p expression by targeting PDGFB genes in OS cells. Conclusion The results demonstrate that resveratrol promotes tumor angiogenesis of OS cells through microRNA-4325p/PDGBB-mediated pathways and provides theoretical and experimental support for the discovery of new bioactive phytochemicals that are effective therapeutic agents in the prevention and treatment of OS diseases.