Cytotoxic Effects of Cucurbitacin I and Ecballium elaterium on Breast Cancer Cells

Abstract

The objective of this study was to investigate the inhibitory effect of cucurbitacin I (CuI) and Ecballium elaterium L. (fruit juice and chloroform extract) on breast cancer cells (MCF-7 and MDA-MB-231). The CuI content of E. elaterium fruit juice and chloroform extract was quantified using high performance liquid chromatography. The cytotoxic effects of the fruit juice, chloroform extract and CuI were determined by MTT, wound healing and colony formation assays; all had an anti-proliferative activity on the breast cancer cells. Clarifying the mechanisms of cucurbitacins will enable the identification of new molecular targets for breast cancer therapy.