Chemical Constituents and Coagulation Activity of Amygdalus persica L. Flowers

Abstract

The ethyl acetate extract of Amygdalus persica L. flowers (family Rosaceae) was fractionated by silica gel and Sephadex LH-20 column chromatography, and, after recrystallization, oleanolic acid (1), ursolic acid (2), quercetin (3), kaempferol (4), hesperetin (5), naringenin (6), and kaempferol-3- O-glucoside (7) were isolated. Their identities were elucidated by spectral techniques and from their physicochemical properties. Compounds 3-7 were identified from A. persica flowers for the first time. All the compounds were evaluated for their procoagulant activity by determining their activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and fibrinogen (FIB) in vitro. The coagulation activity results showed that oleanolic acid and ursolic acid could significantly prolong APTT and TT; quercetin could significantly shorten PT and TT; kaempferol and hesperetin could significantly shorten APTT, PT, and TT; and naringenin could significantly shorten APTT, PT, and TT and decrease the content of FIB compared with the blank group. All of the above revealed that quercetin, kaempferol, hesperetin and naringenin possessed significant procoagulant activity, while ursolic acid had anticoagulant activity in vitro.