Scheduled Voluntary Wheel Running Activity Modulates Free-Running Circadian Body Temperature Rhythms in Octodon degus

Abstract

Entrainment of the circadian pacemaker to nonphotic stimuli, such as scheduled wheel-running activity, is well characterized in nocturnal rodents, but little is known about activity-dependent entrainment in diurnal or crepuscular species. In the present study, effects of scheduled voluntary wheel-running activity on circadian timekeeping were investigated in Octodon degus,a hystricomorph rodent that exhibits robust crepuscular patterns of wakefulness. When housed in constant darkness, O. degus exhibited circadian rhythms in wheel-running activity and body temperature (Tb) with an average period length ([.tau]) of 23.39 ± 0.11 h. When wheel running was restricted to a fixed 2-h schedule every 24 h, [.tau] increased on average 0.39 ± 0.09 h but did not result in steady-state entrainment. Instead, relative coordination between the fixed running schedule and circadian timing was observed. [.tau] was greatest when scheduled wheel running occurred at CT 20.5 (0.4 h greater than DD baseline [.tau]). Scheduled running activity also influenced Tb waveform symmetry, reflecting concomitant changes in the circadian activity-rest ratio ([.alpha]:[.rho]). Aftereffects of the scheduled wheel-running paradigm were also observed. In 2 animals, [.tau] lengthened from 23.20 and 23.80 h to 24.14 and 24.15 h, respectively, and remained relatively stable for approximately 1 month during the wheel schedule. Although behavioral activity appears to be a weak zeitgeber in this species, these data suggest that nonphotic stimuli can phase delay the circadian pacemaker in O. degusat similar times of the day as in nocturnal hamsters and mice, and in humans.