A Role for the Circadian Clock of the Suprachiasmatic Nuclei in the Interpretation of Serial Melatonin Signals in the Syrian Hamster

Abstract

Seasonal rhythms of reproduction in the Syrian hamster are triggered by the pineal hormone melatonin. By varying the parameters of systemic infusions of exogenous melatonin delivered to pinealectomized hamsters, it has been shown that the hypothalamus is sensitive to the duration of individual signals, which serve as an inverse coding of day length. It also has been shown that animals are sensitive to the temporal structure of a series of signals insofar as a series of melatonin infusions of appropriate number and duration may fail to invoke a gonadal response if they are presented at inappropriate frequencies. Although the endogenous circadian pacemaker of the suprachiasmatic nucleus (SCN) is not thought to be involved in the measurement of or response to melatonin signal duration, its contribution to the interpretation of a series of melatonin signals remains to be determined. Syrian hamsters are able to show a short-day-like gonadal response to a series of melatonin signals delivered on a variety of noncircadian schedules, including one in which a "random" pattern of infusions is employed. This study investigated the role of the SCN in the interpretation of such infusion paradigms. Adult male Syrian hamsters received electrolytic lesions of the SCN. Pinealectomized, lesioned, and intact hamsters then were infused with melatonin or saline at one of three different phases of the day in a random pattern such that no signal was predictive of the timing of the next. Other lesioned and intact animals received melatonin or saline at the same time daily After 6 weeks, control saline-infused animals in both lesioned and intact groups had large testes. However, sham animals receiving melatonin in the random infusion pattern had regressed testes, as did the lesioned animals receiving melatonin at the same phase every day By contrast, lesioned animals that received melatonin in the random pattern of infusion did not show a short-day gonadal response. These results suggest that although the SCN is not necessary for measurement of the duration of individual signals, it may play a role in the interpretation of a series of melatonin signals in which the number of melatonin signals and the period of time over which they are encountered need to be compared.