Pigment Dispersing Factor: An Output Regulator of the Circadian Clock in the German Cockroach-Reference-Cited by-同舟云学术

Pigment Dispersing Factor: An Output Regulator of the Circadian Clock in the German Cockroach

Published:2009-02 Issue:1 Volume:24 Page:35-43
ISSN:0748-7304
Container-title:Journal of Biological Rhythms
language:en
Short-container-title:J Biol Rhythms

Author:

Lee Chi-Mei¹,Su Ming-Tsan²,Lee How-Jing³

Affiliation:

1. Department of Entomology, National Taiwan University, Taipei, Taiwan, Department of Life Science, National Taiwan Normal University, Taipei, Taiwan

2. Department of Life Science, National Taiwan Normal University, Taipei, Taiwan

3. Department of Entomology, National Taiwan University, Taipei, Taiwan,

Abstract

Pigment-dispersing factor (PDF) is a neuropeptide that is synthesized specifically and constantly in the circadian clock cells of many insects. The functions of PDF have not been fully determined, but it might serve as the output and coupling signal of circadian locomotor rhythms. In this experiment, we explore the functions of PDF in the German cockroach with RNA interference technique. Since the 2nd day after pdf double-strand RNA (dsRNA) injection, the amount of pdf mRNA decreased significantly, and this knockdown effect could persist at least 56 days. With immunostaining technique, the clock cells of pdf dsRNA-injected cockroaches could not be stained by anti-PDF antibody. In the behavioral study, pdf dsRNA injection caused rhythmic males to become arrhythmic in light-dark cycles or in constant darkness. In addition, due to the nocturnal nature of the German cockroaches, the locomotor activity increased after lights-off or entering subjective night. However, this activity peak gradually disappeared after pdf dsRNA injection. Based on these 2 lines of evidences, PDF serves as an output regulator of locomotor circadian rhythm in the German cockroach.

Publisher

SAGE Publications

Subject

Physiology (medical),Physiology

Link

http://journals.sagepub.com/doi/pdf/10.1177/0748730408327909

Reference36 articles.

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