The Neuropeptide PDF Is Crucial for Delaying the Phase of Drosophila’s Evening Neurons Under Long Zeitgeber Periods

Abstract

Circadian clocks schedule biological functions at a specific time of the day. Full comprehension of the clock function requires precise understanding of their entrainment to the environment. The phase of entrained clock is plastic, which depends on different factors such as the period of endogenous oscillator, the period of the zeitgeber cycle (T), and the proportion of light and darkness (day length). The circadian clock of fruit fly Drosophila melanogaster is able to entrain to a wide range of T-cycles and day lengths. Here, we investigated the importance of the neuropeptide Pigment-Dispersing Factor (PDF) for entrainment by systematically studying locomotor activity rhythms of Pdf 0 mutants and wild-type flies under different T-cycles (T22 to T32) and different day lengths (8, 12, and 16 hour [h]). Furthermore, we analysed PERIOD protein oscillations in selected groups of clock neurons in both genotypes under T24 and T32 at a day length of 16 h. As expected, we found that the phase of Drosophila’s evening activity and evening neurons advanced with increasing T in all the day lengths. This advance was much larger in Pdf 0 mutants (~7 h) than in wild-type flies causing (1) pronounced desynchrony between morning and evening neurons and (2) evening activity to move in the morning instead of the evening. Most interestingly, we found that the lights-off transition determines the phase of evening neurons in both genotypes and that PDF appears necessary to delay the evening neurons by ~3 h to their wild-type phase. Thus, in T32, PDF first delays the molecular cycling in the evening neurons, and then, as shown in previous studies, delays their neuronal firing rhythms to produce a total delay of ~7 h necessary for a wild-type evening activity phase. We conclude that PDF is crucial for appropriate phasing of Drosophila activity rhythm.