Cell death pattern of a varicose vein organ culture model

Author:

Lim Chung S12,Kiriakidis Serafim23,Paleolog Ewa M23,Davies Alun H1

Affiliation:

1. Academic Section of Vascular Surgery, Department of Surgery and Cancer, London, W6 8RF, UK

2. Cytokine Biology of Vessels, Kennedy Institute of Rheumatology, Faculty of Medicine, Imperial College, London, W6 8LH, UK

3. Kennedy Institute of Rheumatology, University of Oxford, London, W6 8LH, UK

Abstract

The study aimed to investigate the viability of a varicose vein (VV) organ culture model by assessing cell death pattern. To assess pattern of cell death with time, VV organ cultures were incubated for up to 14 days with regular medium changed. To assess viability, cell death of VV organ cultures treated with sodium azide and their untreated counterparts was assayed. Increased cell death was measured in VV organ cultures from day 0 to 2. Cell death decreased gradually after day 2 and plateaued from day 8 to 14. VV organ cultures treated with sodium azide demonstrated significantly more cell death in tissue ( P = 0.001). Cell death measured in cultures treated with sodium azide continued to increase until day 7. In conclusion, this study demonstrated the viability of a VV organ culture model with most cell death occurred within the first two days and then declined to a relatively low level.

Publisher

SAGE Publications

Subject

Cardiology and Cardiovascular Medicine,Radiology, Nuclear Medicine and imaging,General Medicine,Surgery

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. In Vitro Assays Used to Analyse Vascular Cell Functions;Fundamentals of Vascular Biology;2019

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