In Vitro Evaluation of 3T3 and MDBK Cells Attachment and Proliferation on Collagen and Fibronectin Immobilized Nonwoven Polylactide Matrices

Abstract

Fibroblast-like, 3T3 (Mouse Swiss Albino) and epithelial-like MDBK (Madine Darby Bovine Kidney) cells were selected as model cell lines and the adhesion of these cells on nonwoven poly DL-lactide (PDLLA) matrices were investigated. PDLLA was synthesized by ring-opening polymerization of DL-lactide. Nonwoven PDLLA matrices were prepared by an extrusion/winding process. Surface modification of these matrices was achieved by glow-discharge treatment, which was followed by glutaraldehyde incorporation. Biologically modification was performed by immobilization of collagen and/or fibronectin for cell attachment and proliferation. Adhesion values of fibroblast-like and epithelial-like cells after incubation for 2 h was 90 and 85% of initial cells, respectively. The 3T3 cells grown on PDLLA, after 11 days of incubation, was 9 105 cells while the control group was 1.2 106 cells. MDBK cells grown on the same polymeric matrices were determined to be 1.5 106 cells and 1.8 106cells for the control group. These results indicate that biodegradable nonwoven PDLLA matrices can be used for adhesion and proliferation of primary cell cultures and that implants of polymer-cells composites can be used in wound healing. An important advantage of these matrices is that a second surgical operation is not necessary to remove the implant due to the biodegradation of polymer in tissue.