In Vitro Alternatives for Ocular Safety Testing: An Outline of Assays and Possible Future Developments

Abstract

An increasing number of in vitro tests are being proposed as alternatives for animals in ocular safety testing. These in vitro systems use a variety of approaches and measure a broad range of endpoints, including cellular cytotoxicity and metabolism. We will briefly review the development and validation of these model systems. Several tests appear promising, and may prove useful as adjuncts in our current ocular safety assessment schemes. We will also discuss how these tests can most rapidly be brought to use in ocular safety assessment, and where efforts should be directed to develop more-highly predictive in vitro model systems for the future.