Frog Skin: A Computer Simulation of Experiments Performed on Frog SkinIn Vitroto Investigate the Epithelial Transport of Ions

Abstract

An interactive computer-assisted learning program is described, based on experiments performed on frog skin in vitro, a preparation commonly used to teach the principles of ion transport across tight epithelia. It is aimed at undergraduate students on a variety of biomedical science courses and is designed for use on any IBM-compatible microcomputer. The program uses data derived from theoretical models to allow students to design experiments by altering certain experimental parameters. They can investigate, for example, the effects of changing the concentrations of certain ions on either side of the skin or the actions of certain drugs on either passive or active transport. Such investigations involve taking measurements from a simulated voltmeter, ammeter or radiation (scintillation) counter, as appropriate. The complete learning package includes background information for the student, tutor's notes, and suggested student assignments. The use of the program in teaching physiology and its value as an alternative to animal experiments are discussed.