Hydrogen Gas Inhalation Attenuates Acute Impulse Noise Trauma: A Preclinical In Vivo Study

Author:

Videhult Pierre Pernilla1ORCID,Fransson Anette23,Kisiel Marta A.4,Laurell Göran2

Affiliation:

1. Division of Audiology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Sweden

2. Department of Surgical Sciences, Uppsala University Hospital, Uppsala, Sweden

3. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

4. Department of Medical Sciences, Occupational and Environmental Medicine, Uppsala University Hospital, Uppsala, Sweden

Abstract

Objective: Molecular hydrogen (H2) has shown therapeutic potential in several oxidative stress-related conditions in humans, is well-tolerated, and is easily administered via inhalation.The aim of this preclinical in vivo study was to investigate whether impulse noise trauma can be prevented by H2 when inhaled immediately after impulse noise exposure. Methods: Guinea pigs (n = 26) were subjected to impulse noise (n = 400; 156 dB SPL; 0.33/s; n = 11; the Noise group), to impulse noise immediately followed by H2 inhalation (2 mol%; 500 ml/min; 1 hour; n = 10; the Noise + H2 group), or to H2 inhalation (n = 5; the H2 group). The acoustically evoked ABR threshold at 3.15, 6.30, 12.5, 20.0, and 30.0 kHz was assessed before and 4 days after impulse noise and/or H2 exposure. The cochleae were harvested after the final ABR assessment for quantification of hair cells. Results: Noise exposure caused ABR threshold elevations at all frequencies (median 35, 35, 30, 35, and 35 dB SPL, the Noise group; 20, 25, 10, 13, and 20 dB SPL, the Noise + H2 group; P < .05) but significantly less so in the Noise + H2 group ( P < .05). Outer hair cell (OHC) loss was in the apical, mid, and basal regions 8.8%, 53%, and 14% in the Noise group and 3.5%, 22%, and 1.2% in the Noise + H2 group. The corresponding inner hair cell (IHC) loss was 0.1%, 14%, and 3.5% in the Noise group and 0%, 2.8%, and 0% in the Noise + H2 group. The difference between the groups was significant in the basal region for OHCs ( P = .003) and apical ( P = .033) and basal ( P = .048) regions for IHCs. Conclusions: Acute acoustic trauma can be reduced by H2 when inhaled immediately after impulse noise exposure.

Funder

the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement

AFA Försäkring

Stiftelsen Tysta Skolan

Publisher

SAGE Publications

Subject

General Medicine,Otorhinolaryngology

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