Inflammatory Response to Chronic Otitis Media in Digeorge Syndrome: A Case Study Using Immunohistochemistry on Archival Temporal Bone Sections

Author:

Ganbo Tetsuya1,Sando Isamu2,Suzuki Chiaki2,Balaban Carey D.2,Kitagawa Miwa2

Affiliation:

1. Tokyo, Japan

2. Pittsburgh, Pennsylvania

Abstract

Immunohistochemical analyses were conducted on archival celloidin-embedded human temporal bone sections from an 8-month-old boy with chronic otitis media and DiGeorge syndrome. We employed antigen retrieval methods with saturated sodium hydroxide—methanol solution, microwave incubation, and proteolytic treatment to demonstrate the distribution of T-lymphocytes, B-lymphocytes, macrophages, and intercellular adhesion molecule 1 (ICAM-1) expression in the middle ear. B-lymphocytes and macrophages were observed predominantly within the middle ear mucosa. T-lymphocytes were rare. Further, ICAM-1 was expressed in the vascular endothelium of the lamina propria, as well as infiltrating mononuclear cells. This suggests that the expression of ICAM-1 can be induced in the middle ear with otitis media, even if T-lymphocytes are depressed in a cell-mediated immunodeficiency disorder such as DiGeorge syndrome.

Publisher

SAGE Publications

Subject

General Medicine,Otorhinolaryngology

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