Salivary HPV Persistence Following Treatment of Oropharyngeal Squamous Cell Carcinoma

Author:

Quimby Alexandra E.1ORCID,Lagiou Pagona23,Purgina Bibiana4,Corsten Martin5,Johnson-Obaseki Stephanie1

Affiliation:

1. Department of Otolaryngology – Head & Neck Surgery, University of Ottawa, Ottawa, ON, Canada

2. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA

3. School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

4. Division of Anatomical Pathology, Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada

5. Division of Otolaryngology – Head & Neck Surgery, Department of Surgery, Dalhousie University, Halifax, NS, Canada

Abstract

Objective: To determine the persistence of human papillomavirus (HPV) infection following treatment of HPV-positive oropharyngeal squamous cell carcinoma (HPV + OPSCC). Methods: A cross-sectional study was undertaken at The Ottawa Hospital (Ottawa, ON, Canada), a tertiary academic hospital and regional cancer center. Adult patients who were diagnosed with HPV + OPSCC between the years of 2014 and 2016 and treated with curative intent, and who were alive and willing to consent were eligible for inclusion. A saliva assay was used to test for the presence of HPV DNA in a random sample of patients. qPCR was used to amplify DNA from saliva samples. Results: Saliva samples were obtained from 69 patients previously treated with HPV + OPSCC. All patients had a minimum of 2 years of follow-up. 5 patients tested positive for HPV: 2 were positive for HPV-16, 2 for HPV-18, and 1 “other” HPV type. No patient in our study cohort had suffered recurrence post-treatment. Conclusions: This study is the first to demonstrate the prevalence of persistent oncogenic HPV DNA in saliva following treatment for HPV + OPSCC. This prevalence appears to be low, despite the fact that persistent HPV infection is a precursor for the development of HPV + OPSCC. This finding raises questions about what factors influence the clearance or persistence of HPV DNA in saliva after treatment for HPV + OPSCC, and may add to our understanding about the longitudinal effects of HPV infection in these cancers.

Publisher

SAGE Publications

Subject

General Medicine,Otorhinolaryngology

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