Affiliation:
1. From the Yamins Laboratory for Surgical Research, Beth Israel Hospital, the Department of Surgery, Harvard Medical School, and the Chemical Laboratories of Harvard University, Boston, Massachusetts
Abstract
The synthesis of a substrate, 6-bromo-2-naphthyl β-d-glucopyruronoside, is described, and the procedure for the histochemical demonstrations of β-d-glucuronidase activity and experiments to determine the reliability of the results of localization of enzymatic activity are given. The results in normal tissues of the rat are presented. Greatest concentration of enzymatic activity was seen in liver, spleen, epithelium of gastrointestinal tract, uterus, thyroid, and in the white matter of the nervous system.
Cited by
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