The Endoplasmic Reticulum-Mitochondria Encounter Structure Complex Coordinates Coenzyme Q Biosynthesis

Author:

Eisenberg-Bord Michal1,Tsui Hui S.2,Antunes Diana3,Fernández-del-Río Lucía2,Bradley Michelle C.2,Dunn Cory D.45,Nguyen Theresa P. T.6,Rapaport Doron3,Clarke Catherine F.2,Schuldiner Maya1

Affiliation:

1. Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel

2. Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, CA, USA

3. Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany

4. Institute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland

5. Department of Molecular Biology and Genetics, Koç University, İstanbul, Turkey

6. Department of Chemistry, Loyola University Maryland, Baltimore, MD, USA

Abstract

Loss of the endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) complex that resides in contact sites between the yeast ER and mitochondria leads to impaired respiration; however, the reason for that is not clear. We find that in ERMES null mutants, there is an increase in the level of mRNAs encoding for biosynthetic enzymes of coenzyme Q6 (CoQ6), an essential electron carrier of the mitochondrial respiratory chain. We show that the mega complexes involved in CoQ6 biosynthesis (CoQ synthomes) are destabilized in ERMES mutants. This, in turn, affects the level and distribution of CoQ6 within the cell, resulting in reduced mitochondrial CoQ6. We suggest that these outcomes contribute to the reduced respiration observed in ERMES mutants. Fluorescence microscopy experiments demonstrate close proximity between the CoQ synthome and ERMES, suggesting a spatial coordination. The involvement of the ER-mitochondria contact site in regulation of CoQ6 biogenesis highlights an additional level of communication between these two organelles.

Funder

National Institute of General Medical Sciences

H2020 European Research Council

Volkswagen of America

Deutsche Forschungsgemeinschaft

European Molecular Biology Organization

Division of Molecular and Cellular Biosciences

Publisher

SAGE Publications

Subject

General Materials Science

Cited by 38 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3