Leukocyte Tissue-Type Plasminogen Activator Activity in Dialysis Patients

Author:

Nishimoto K1,Kishimoto T.1,Yamagami S.1,Sugimura T.1,Mori K.1,Tanaka H.1,Maekawa M.1,Okada K.2,Matsuo O.2

Affiliation:

1. Department of Urology, Osaka City University Medical School, Osaka - Japan

2. Department of Physiology, Kinki University Medical School, Osaka - Japan

Abstract

Plasma fibrinopeptide Bβ15–42 was significantly high in undialyzed and hemodialyzed chronic renal failure (CRF) patients, indicating that the fibrinolytic system as well as the coagulation system is stimulated. There are two kinds of plasminogen activators (PA) for the fibrinolytic system: urokinase (UK) and tissue-type PA (t-PA). PA activity of peripheral leukocytes from healthy volunteers, continuous ambulatory peritoneal dialysis (CAPD) patients and hemodialysis (HD) patients was measured and compared. Peripheral leukocyte PA in the euglobulin fraction was purified using zinc-chelate-Sepharose 6B column and Concanavalin A-Sepharose column chromatography. The different PA activity was quantitatively identified by electrophoretic enzymography and was confirmed using antibody against UK and t-PA. PA activity of peripheral leukocytes was significantly higher in HD patients on the cupro-ammonium processing membrane dialyzer than in CAPD patients and healthy volunteers. All PA activity in the three groups was UK, and t-PA was not detected. This suggested that the inflammatory response was continuously induced in HD patients, resulting in the induction of PA activity of the leukocytes and plasma, and that different mechanisms were involved for the synthesis or secretion of UK and t-PA.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Risk Factors of Ischemic Cardiac Disease in Patients on Continuous Ambulatory Peritoneal Dialysis;Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis;1993-01

2. Risk Factors of Ischemic Cardiac Disease in Patients on Continuous Ambulatory Peritoneal Dialysis;Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis;1993-01

3. Evidence for the role of dialysis hypoxemia in the pathogenesis of hemodialysis-induced rise in tissue-type plasminogen activator;Thrombosis Research;1992-09

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