Conditions Required for a Hybrid Artificial Liver Support System using a PUF/Hepatocyte-Spheroid Packed-Bed Module and it's use in Dogs with Liver Failure-Reference-Cited by-同舟云学术

Conditions Required for a Hybrid Artificial Liver Support System using a PUF/Hepatocyte-Spheroid Packed-Bed Module and it's use in Dogs with Liver Failure

Published:2000-07 Issue:7 Volume:23 Page:446-453
ISSN:0391-3988
Container-title:The International Journal of Artificial Organs
language:en
Short-container-title:Int J Artif Organs

Author:

Ijima H.¹,Nakazawa K.¹,Koyama S.¹,Kaneko M.¹,Matsushita T.¹,Gion T.²,Shirabe K.²,Shimada M.²,Takenaka K.²,Sugimachi K.²,Funatsu K.¹

Affiliation:

1. Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University

2. Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka - Japan

Abstract

We studied the effects of a hybrid artificial liver support system we developed on dogs with hepatic failure. The system consisted of a multi-channel polyurethane foam packed-bed culture module, including primary dog hepatocyte spheroids. Blood ammonia was well metabolized by 20 g hepatocytes, but the other functions such as glucose concentration, total bile acid concentration, and survival time required 30 g hepatocytes to improve conditions. We found that we should use a culture substratum that easily forms spheroids, and that an artificial liver module should be used as soon as possible after spheroid formation by hepatocytes in the module.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

Link

http://journals.sagepub.com/doi/pdf/10.1177/039139880002300707

Reference24 articles.

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3. Entrapment of Hepatocyte Spheroids in a Hollow Fiber Bioreactor as a Potential Bioartificial Liver

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