Nonenzymatic versus Enzymatic Hepatocyte Isolation from Pig Livers for Larger Scale Investigations of Liver Cell Perfusion Systems-Reference-Cited by-同舟云学术

Nonenzymatic versus Enzymatic Hepatocyte Isolation from Pig Livers for Larger Scale Investigations of Liver Cell Perfusion Systems

Published:1993-09 Issue:9 Volume:16 Page:677-681
ISSN:0391-3988
Container-title:The International Journal of Artificial Organs
language:en
Short-container-title:Int J Artif Organs

Author:

Gerlach J.C.¹,Brombacher J.¹,Courtney J.M.²,Neuhaus P.¹

Affiliation:

1. Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin - Germany

2. Bioengineering Unit, University of Strathclyde, Glasgow - UK

Abstract

A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 ± 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

Link

http://journals.sagepub.com/doi/pdf/10.1177/039139889301600909

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