rhVEGF and Experimental Rat Skin Flaps: Systemic or Local Administration and Morphological Characteristics-Reference-Cited by-同舟云学术

rhVEGF and Experimental Rat Skin Flaps: Systemic or Local Administration and Morphological Characteristics

Published:2001-10 Issue:10 Volume:24 Page:743-751
ISSN:0391-3988
Container-title:The International Journal of Artificial Organs
language:en
Short-container-title:Int J Artif Organs

Author:

Tucci M.G.¹,Scalise A.²,Lucarini G.³,Pierangela M.²,Pugnaloni A.³,Bertani C.⁴,Orlando F.⁵,Aldini N. Nicoli⁶,Santoli M.²,Campodonico A.²,Gavaudan F.²,Ricotti G.¹,Bertani A.¹,Biagini G.¹

Affiliation:

1. U.O. Dermatologia, I.N.R.C.A. - IRCSS, Ancona - Italy

2. Clinica di Chirurgia Plastica e Ricostruttiva, Università degli Studi di Ancona, Ancona - Italy

3. Istituto di Morfologia Umana Normale, Università degli Studi di Ancona, Ancona - Italy

4. Istituto di Clinica Chirurgica Veterinaria e Medicina di Urgenza, Università degli Studi di Parma, Parma - Italy

5. Dipartimento Ricerche, I.N.R.C.A. - IRCSS, Ancona - Italy

6. Experimental Surgery Department, Research Institute Codivilla Putti I.O.R. - Rizzoli Ortopaedic Institute, Bologna - Italy

Abstract

Skin flap survival is a significant problem in skin surgery; in particular, inadequate arterial or venous blood supply results in necrosis of the distalmost portion. The aim of this study was to evaluate the ability of Vascular Endothelial Growth Factor (VEGF) of modifying the morphological features of skin flaps. Bilateral epigastric skin flaps were raised in 16 Wistar male rats. The epigastric artery and vein of the left flaps were clamped and then injected with rhVEGF (8 rats) or saline (8 rats). The right flaps were not clamped and received rhVEGF or saline systemically. The rats were euthanized on the seventh day and flap skin samples collected. Tissue fragments were subject to immunohistochemical (rhVEGF, VEGFr, VIII factor, CD34 antibodies), ultrastructural and morphostructural investigations. The results showed that rhVEGF improved the condition of flaps and that systemic administration was effective in promoting the development of an adequate vascular network.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

Link

http://journals.sagepub.com/doi/pdf/10.1177/039139880102401007

Reference18 articles.

1. Purification of a glycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line.

2. Keratinocyte-Derived Vascular Permeability Factor (Vascular Endothelial Growth Factor) Is a Potent Mitogen for Dermal Microvascular Endothelial Cells

3. The vascular endothelial growth factor family of polypeptides

4. Enhanced Vascularization of Cultured Skin Substitutes Genetically Modified to Overexpress Vascular Endothelial Growth Factor11The authors declared in writing to have no conflict of interest.

5. The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF.

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