Effect of Gamma Sterilisation and Deep-Freezing on Length and Strength of Fascia Latae

Author:

Kayurapan Agawit1,Makadelok Sahachart1,Waikakul Saranatra1

Affiliation:

1. Bangkok Biomaterial Center, Department of Orthopaedic Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand

Abstract

Purpose. To compare the length and strength of fascia latae after gamma sterilisation and different durations of deep-freezing. Methods. 50 pieces of fresh porcine fascia latae were randomly divided into 5 groups. Group 1 acted as controls, which were not gamma irradiated and deep-frozen. In groups 2 to 4, fascia latae were incubated in phosphate buffer solution for 4 hours, and then gamma irradiated at 25 kGy. They were preserved at −70°C for one to 3 months, respectively. In group 5, fascia latae were preserved for 3 months, and during the whole process they were fixed on a wooden board to maintain their original length. The maximum tensile strength of each fascia lata was tested at a displacement rate of 1 cm per minute until failure. Results. The maximum tensile strength was not significantly different among groups 1 to 4, but was significantly higher in group 5. Conclusion. Gamma sterilisation and deep-freezing had no effect on the strength of fascia latae, but fixation on a board could increase strength.

Publisher

SAGE Publications

Subject

Surgery

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