Affiliation:
1. Laboratory of Cellular Neuropathology, Harvard Medical School, Boston, Massachusetts
Abstract
Polyvinyl alcohol is a highly polar, water-soluble resin. Fixed, washed specimens are soaked in a mixture of 15% polyvinyl alcohol, 15% glycerol and 70% water, which is then allowed to dry at room temperature to a hard, sectionable block. Sections 1-100 µ thick are floated on water, dried on glass slides and stained. There is good preservation of tissue and cell structure, and of many lipids and enzymes.
Cited by
10 articles.
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