Structural Reassignment of Koetjapic Acid following X-ray Crystallography and NMR Spectroscopy

Abstract

The crystal structure and absolute configuration of koetjapic acid were unambiguously reassigned by X-ray crystallography with strong support from NMR spectroscopic data. The acid contained 9 quaternary carbon atoms existing as an orthorhombic crystal with a space group of P21 21 21 and unit cell parameters of a = 7.6641(2), b = 14.6844(4) and c = 23.9316(6). Ring A adopted a chair conformation, ring B has an envelope conformation, whilst ring C assumed a half-chair and D displayed a chair conformation. The absolute configurations at C1 ( R), C5 ( R), C7 ( S), C10 ( S), C13 ( R), C14 ( R), C17 ( S) and C18 ( S) were assigned for the first time. The X-ray crystal of koetjapic acid was therefore reassigned as 3,4-seco-olean-4(23),12-diene-3,30-dioic acid. A plausible biogenetic synthetic pathway for compound 1 is also proposed.