Analysis on the Co-Localization of Asbestos Bodies and Fas or CD163 Expression in Asbestos Lung Tissue by in-Air Micro-Pixe

Author:

Matsuzaki S.1,Shimizu Y.12,Dobashi K.3,Nagamine T.4,Satoh T.5,Ohkubo T.5,Yokoyama A.5,Ishii Y.5,Kamiya T.5,Arakawa K.5,Makino S.2,Utsugi M.1,Ishizuka T.1,Tanaka S.6,Shimizu K.7,Mori M.1

Affiliation:

1. Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Gunma

2. World Health Organization Collaborating Center of Prevention and Control of Chronic Respiratory Diseases/Dokkyo University, Tochigi

3. Gunma University Faculty of Health Science, Gunma

4. 21st Century COE Program, Gunma University Faculty of Health Science, Gunma

5. Japan Atomic Energy Agency, Takasaki Advanced Radiation Research Institute, Takasaki, Gunma

6. Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma

7. Divisiont of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Gunma, Japan

Abstract

To prevent and control diseases caused by exposure to various agents, it is necessary to determine the harmful level for intervention and to establish a method for measuring that level. In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is based on irradiation of specimens with a proton ion microbeam, and has been modified for biological application. Two-dimensional analysis and quantitative analysis using the system confirmed that asbestos induced apoptosis by upregulating Fas expression and also revealed the accumulation of CD163-expressing macrophages in the lungs of patients with asbestosis. By quantitative comparison of the area of Fas or CD163 expression and the Fas- or CD163-negative area in asbestos lung tissue, the harmful levels which caused the expression of Fas or CD163 could be estimated on Silica, Ferrous iron, and Magnesium (the components of asbestos) deposition. These results indicate that the system could be useful for investigating the pathogenesis of inhaled particle-induced immune reactions and for determining harmful levels of exogenous agents.

Publisher

SAGE Publications

Subject

Pharmacology,Immunology,Immunology and Allergy

Cited by 13 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3