The Use of Immunoperoxidase in the Detection of the Erysipelothrix Insidiosa Antigen in Experimentally Infected Turkeys

Author:

Vlemmas I.1,Papaioannou N.1,Frydas S.2,Tsangaris Th.1

Affiliation:

1. Department of Pathology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54006, Greece

2. Department of Parasitology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54006, Greece

Abstract

A simple method using direct and indirect polyclonal antibody-based immunoperoxidase techniques were used to detect E. insidiosa antigen in various organ specimens (kidney, heart, skin, joint of knee and bursa of Fabricius) in experimentally infected turkeys. The specimens were fixed in formalin and ethanol and then embedded in paraffin. By using the above methods E. insidiosa bacilli were detected intracellularly in macrophages and endothelial cells of the vessels. They were also found extracellularly in the lumen of the vessels, either free or in aggregations of bacteria trapped in fibrin thrombi. It is interesting to note the presence of the E. insidiosa bacillus antigen in the cytoplasm of the endothelial cells of the vessels, which indicates intense phagocytic activity.

Publisher

SAGE Publications

Subject

Pharmacology,Immunology,Immunology and Allergy

Reference15 articles.

1. Experimenteller Rotlauf der Puten nach intravenöser Infektion mit Erysipelothrix insidiosa I. Elektronenmikroskopische Befunde in den Nieren

2. Pathology of Experimental Erysipelas in Turkeys

3. Rosenwald A.S., Corstvet R.E., 1978. In Disease of Poultry 6th ed, Iowa State Univ. Press, ed. Hofstad M.S. Ames, Clanek B.W., Helboldt C.F., Reid W.M., Yoder H.W., p. 348.

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