A rationally designed peptide IA-2-P2 against type 1 diabetes in streptozotocin-induced diabetic mice-Reference-Cited by-同舟云学术

A rationally designed peptide IA-2-P2 against type 1 diabetes in streptozotocin-induced diabetic mice

Published:2017-03-01 Issue:3 Volume:14 Page:184-190
ISSN:1479-1641
Container-title:Diabetes and Vascular Disease Research
language:en
Short-container-title:Diabetes and Vascular Disease Research

Author:

Shen Lili¹,Lu Shiping¹,Huang Dongcheng¹,Li Guoliang¹,Liu Kunfeng¹,Cao Rongyue¹,Zong Li²,Jin Liang¹,Wu Jie¹

Affiliation:

1. Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China

2. Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, China

Abstract

Recent studies have investigated the potential of type 1 diabetes mellitus–related autoantigens, such as heat shock protein 60, to induce immunological tolerance or to suppress the immune response. A functional 24-residue peptide derived from heat shock protein 60 (P277) has shown anti-type 1 diabetes mellitus potential in experimental animals and in clinical studies, but it also carries a potential atherogenic effect. In this study, we have modified P277 to retain an anti-type 1 diabetes mellitus effect and minimize the atherogenic potential by replacing the P277 B epitope with another diabetes-associated autoantigen, insulinoma antigen-2 (IA-2), to create the fusion peptide IA-2-P2. In streptozotocin-induced diabetic C57BL/6J mice, the IA-2-P2 peptide displayed similar anti-diabetic effects to the control P277 peptide. Also, the IA-2-P2 peptide did not show atherogenic activity in a rabbit model. Our findings indicate the potential of IA-2-P2 as a promising vaccine against type 1 diabetes mellitus.

Funder

National Natural Science Foundation of China

Publisher

SAGE Publications

Subject

Cardiology and Cardiovascular Medicine,Endocrinology, Diabetes and Metabolism,Internal Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1479164116664189

Reference25 articles.

1. Antigen-Based Vaccination and Prevention of Type 1 Diabetes

2. Antigen-Based Therapy for the Treatment of Type 1 Diabetes

3. Vaccination against autoimmune mouse diabetes with a T-cell epitope of the human 65-kDa heat shock protein.

4. Induction and therapy of autoimmune diabetes in the non-obese diabetic (NOD/Lt) mouse by a 65-kDa heat shock protein.

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