Microbial distribution characteristics related to carbon cycle and their potential impact on methanogenesis of coal reservoirs in underground in situ environments

Abstract

Microorganisms are one of the main driving forces of the cycle of carbon and other life elements in the underground environment. The natural environment is the comprehensive result of these microorganisms. In contrast, the study of coal reservoir microorganisms is mostly under laboratory conditions, which limits people's understanding of the symbiotic relationship between microorganisms, the interaction between microorganisms and the environment, and the distribution differences of microbial communities in the region. Similarly, the carbon cycle of the underground environment driven by essential microorganisms in coal reservoirs cannot be further studied. The geochemical process of underground methane generation and oxidation is critical in discussing the production and consumption of biomethane in the underground environment and the metabolic behavior of microorganisms. For this reason, we conducted biogeochemical tests and microbial sequencing on the water produced by coalbed methane wells in the south of the Qinshui Basin to analyze and improve the understanding of the distribution difference and metabolic behavior of microbial communities in coal reservoirs. The concentration of Cl− and HCO3− in the detention environment in the study area increases, while the concentration of SO42−, NO3−, NO2−, and Fe3+ decreases with the increase of coal seam depth, reflecting the distribution difference of hydrochemical environment and redox conditions of the underground reservoir in the study area. The results of microbial sequencing showed microbial methanogenesis in the study area, but it could also be consumed by microbial oxidation simultaneously. The microbial communities related to methane production and consumption had diversity distributions similar to geochemical parameters and geographical patterns. Methanogens and dissolved inorganic carbon isotopes confirmed the potential of in situ methane generation. Still, biomethane's enrichment and accumulation conditions and the impact of aerobic/anaerobic oxidation of methane need further study.