Affiliation:
1. Istituto di Immunologia e Malattie Infettive - Università di Verona
Abstract
The application of specific and proven guidelines is required for flow cytometric nuclear DNA analysis, which can provide precise information on genomic quantitative modifications and the proliferative activity of tumour tissues, clinically useful for prognosis and monitoring of therapy and recurrencies. The authors review the various protocol phases to be followed for a correct analysis and discuss the main problems that can be encountered in the procedural steps and in interpreting the histograms.